PROSPER is an integrated feature-based webserver for in silico prediction of protease substrates and their cleavage sites for twenty-four different protease types, covering four major protease families- Aspartic (A), Cysteine (C), Metallo (M) and Serine (S).

Important Notice: You have been redirected from to this new PROSPER server. Kindly update your bookmarks to:

Please input the protein sequence of a substrate in the FASTA format below (example):


ID: (optional, your given name of the sequence)

Email address: (mandatory, where results will be sent to)




Important Notice: We rely on good outcomes from the use of the bioinformatics tools that we have developed for funding and other important considerations. To continue this high-quality service and to enable the further development of cutting-edge tools that meet users' needs, we ask you to consider citing the publication on PROSPER if you get great outcomes from using our service:
Song J, Tan H, Perry AJ, Akutsu T, Webb GI, Whisstock JC and Pike RN. PROSPER: an integrated feature-based tool for predicting protease substrate cleavage sites. PLoS ONE, 2012, 7(11)
: e50300.


Copyright © 2012-2018. Monash Bioinformatics Platform, School of Biomedical Sciences, Faculty of Medicine, Monash University, Australia